LOCUS       Exported                3682 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0058_sensor_plasRstRBS-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="spacer (B0040)"
     promoter        197..353
                     /note="P(LasR) (R0079)"
     RBS             362..373
                     /note="RBS (B0034)"
     CDS             380..1105
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1108..1224
                     /note="T1"
     terminator      1117..1203
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1231..1250
                     /note="AB179"
     primer_bind     1416..1435
                     /note="AB5"
     rep_origin      1569..2396
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1817..1819
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1820..2364)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2468..2487
                     /note="AB180"
     oriT            2498..2606
                     /note="incP origin of transfer"
     CDS             complement(2762..3577)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga
      181 ggtcactgac ctaacagccc ctcgctgagc gcgtcccgga gctgggggca acctagctgc
      241 cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg aaatcaaggc
      301 tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa agctactaga
      361 gaaagaggag aaatactaga tgagcaaagg agaagaactt ttcactggag ttgtcccaat
      421 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtccgtg gagagggtga
      481 aggtgatgct acaaacggaa aactcaccct taaatttatt tgcactactg gaaaactacc
      541 tgttccgtgg ccaacacttg tcactactct gacctatggt gttcaatgct tttcccgtta
      601 tccggatcac atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      661 ggaacgcact atatctttca aagatgacgg gacctacaag acgcgtgctg aagtcaagtt
      721 tgaaggtgat acccttgtta atcgtatcga gttaaagggt attgatttta aagaagatgg
      781 aaacattctt ggacacaaac tcgagtacaa ctttaactca cacaatgtat acatcacggc
      841 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg
      901 ttccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      961 tttaccagac aaccattacc tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa
     1021 gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac atggcatgga
     1081 tgagctctac aaaaggcctt aataaattaa aggcatcaaa taaaacgaaa ggctcagtcg
     1141 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1201 aatccgccgc cctagacagc tgggcgcgcc aaatacgccc ggtagtgatc ttatttcatt
     1261 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     1321 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     1381 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta
     1441 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
     1501 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc
     1561 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     1621 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     1681 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     1741 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     1801 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     1861 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1921 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     1981 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     2041 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     2101 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     2161 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     2221 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     2281 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     2341 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     2401 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2461 atttccccga aaagtgccac ctgctagggc cggcctacgg ccagcctcgc agagcaggat
     2521 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
     2581 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     2641 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     2701 tcgctataat gaccccgaag cagggttatg cagcggaaaa ggacccttgt ccaattaatt
     2761 attagaaaaa ttcatccagc atcagatgaa attgcagttt gttcatatcc ggattatcaa
     2821 tgccatattt ctgaaacaga cgtttttgca ggctcgggct aaattcgccc aggcagttcc
     2881 acagaatggc cagatcctga taacgatccg caatgcccac acggcccaca tcaatgcagc
     2941 caatcagttt gccttcatcg aaaatcaggt tatccaggct aaaatcgccg tgggtcacca
     3001 cgctatccgg gctaaacggc agcagtttat gcatttcttt ccacacctgt tccaccggcc
     3061 agccgttacg ttcatcatca aaatcgctcg catccaccag gccgttgttc atacggctct
     3121 gcgcctgggc cagacgaaac acacgatcgc tgttaaacgg gcagttgcac accggaatgc
     3181 tatgcagacg acgcagaaac acggccagcg catccacaat gttttcgccg ctatccggat
     3241 attcttccag cacctgaaac gcggttttgc ccggaatcgc ggtggtcagc agccacgcat
     3301 catccggggt gcgaataaaa tgtttaatgg tcggcagcgg cataaattcg gtcagccagt
     3361 tcagacgcac catttcatcg gtcacatcgt tcgccacgct gcctttgcca tgtttcagaa
     3421 acagttccgg cgcatccggt ttgccataca gacgataaat ggtcgcgccg ctctgaccca
     3481 cgttatcacg cgcccattta tagccataca gatccgcatc catgttgctg ttcagacgcg
     3541 gacggctaca gctcgtttca cgctgaatat ggctcataac accccttgta ttactgttta
     3601 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3661 agagattttg agacacaaat tt
//
